Specific micro-radioimmunoassay for prednisolone in serum.
نویسندگان
چکیده
Use of an 125I radioimmunoassay involving antiserum coupled to magnetizable cellulose, after prednisolone-21-phosphate interference is removed by dichloromethane extraction at pH 7.4 and endogenous glucocorticoid interference is removed by selective chemical derivatization with Girard's Reagent T [(carboxymethyl)trimethylammonium chloride hydrazide], allows determination of prednisolone in 10 microL of serum. Results correlate well with those of an established liquid-chromatographic method for separating prednisolone from its metabolites.
منابع مشابه
DEVELOPMENT OF A RAPID AND SENSITIVE RADIOIMMUNOASSAY FOR MEASUREMENT OF AFLATOXIN B 1 IN BIOLOGICAL SAMPLES
Aflatoxin B I (AFB) isa well known hepatocarcinogen in several animal species and probably a causative agent in human hepatocellular carcinoma. Humans are exposed to AFB by ingesting contaminated food. Aflatoxin contamination encountered in human foods is usually at low levels which is difficult to measure by chromatographic methods. Therefore in the present study we have developed an immun...
متن کاملEXTENDED REPORT Possible role of leptin in hypoandrogenicity in patients with systemic lupus erythematosus and rheumatoid arthritis
Background: Hypoandrogenicity is common in obesity and in chronic inflammatory diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Adrenal androgens such as androstenedione (ASD) and dehydroepiandrosterone (DHEA) sulphate are low, which partly depends on the influence of TNF in chronic inflammatory diseases. Leptin is stimulated by TNF and is associated with hypoa...
متن کاملStatistical survey of "saturation analysis" calibration curve data for prednisolone, prednisone and digoxin.
An extensive survey of radioimmunoassay calibration data for prednisolone, prednisone and digoxin indicated that the common practice of preparing calibration curves with individual subject's pre-dose plasma or serum, and using this to estimate unknown concentrations for the same subject, is not supported by statistical considerations. Preparation of calibration plots from pooled data is better ...
متن کاملEffect of oral glucocorticoid treatment on serum inflammatory markers in acute asthma.
BACKGROUND Acute asthma is associated with elevated serum concentrations of products of activated T cells and eosinophils. AIMS To compare the changes in concentrations of these products with disease severity and changes in lung function following oral prednisolone treatment. METHODS Twenty patients (mean age 8.7 years) were recruited on admission with acute asthma to a district general hos...
متن کاملOral prednisone for chronic active liver disease: dose responses and bioavailability studies.
Serum concentrations of prednisolone were measured by radioimmunoassay after the administration of prednisone (10, 20, or 30 mg) by mouth to five healthy volunteers, five patients with severe chronic active liver disease (CALD), and five patients with CALD in remission induced by prednisone. Only minor differences were found between the groups and bioavailability was linearly related to the dos...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 29 7 شماره
صفحات -
تاریخ انتشار 1983